Part:BBa_K2448006
PsiR from Agrobacterium tumefaciens
This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743).
Usage and Biology
PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).
Our team used this transcription factor together with the Agrobacterium tumefaciens pPsiA or pPsiR promoters (BBa_K2448010 and BBa_K2448011) as well as with the pPsiTac1 synthetic promoter (BBa_K2448016) to build three Psicose biosensors (BBa_K2448025, BBa_K2448026 and BBa_K2448027). The results show that PsiR behaved as predicted under and without induction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4
Illegal AgeI site found at 691 - 1000COMPATIBLE WITH RFC[1000]
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